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| Product Name: |
Recombinant Bovine Enterokinase
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| Supply Ability: |
100000 |
| Related proudcts |
recombinant, |
| Specifications |
100U�00U�KU�0KU |
| Price Term: |
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| Port of loading: |
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| Minimum Order |
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Recombinant Enterokinase
Catalog Number:REK08
CAS:9017-74-8
EC:3.4.21.9
Storage temperature: -20°C or below
Source: Recombinant enterokinase is a genetically engineered protein expressed in E.coli
Protein Sequence:The amino acid sequence of recombinant enterokinase is identical to bovine enterokinase light chain
1.DESCRIPTION
YaxinBio Enterokinase is a kind of highly purified recombinant bovine enterokinase light chain. The enzyme has been extensively purified by HPLC and there are no traces of other contaminating proteases. Enterokinase specifically hydrolyzes peptide bond at the carboxyl side of lysine residue preceded by four a***rtic acids: Asp-Asp-Asp-Asp-Lys (DDDDK). So, enterokinase can remove N-terminal fusion protein or tags to get aim protein with native amino acids sequence.
2.MAIN FEATURES
Source Recombinant E. coli
Appearance Clear,colorless to yellowish liquid
Activity � u/μl
Purity(SDS-PAGE) Single major band
Molecular Weight(SDS-PAGE) 25.8 ±2.58 kDa
UNIT DEFINITION：One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 12 to16 hours to get 95% completion at 25°C in 25mMTris-HCl, pH 8.0. Substrate: a special fusion protein.Â
3.RECOMMEND USAGE CONDITION:
1) Cutting condition: 25mM Tris-HCl 8.0
Fusion protein concentration: 0.1-1mg/ml (total protein content: 50-100ug)
EK content: 0.1-0.2U
Temperature: 25â„?
Time: overnight or 12h-16h for digestion.
If digestion under 2-8°C,prolong the digestion time two folds,or more enzyme added to short the digestion time.
2) 4°C low temperature digestion conditions:
4°C can effectively digest the substrate, but need to extend the digestion time to 48-64h, or increase the amount of 2-3 times the amount of enzyme.
3) Enzyme cleavage optimization and amplification
Optimization: Enzyme cleavage conditions can be optimized, such as buffer pH, fusion protein concentration, EK amou |
| Company: |
Shanghai Yaxin Biotechnology Co., Ltd.
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| Contact: |
Mr. Zhi Zhao |
| Address: |
Room 101, Building 1, No. 466 Yin Du Road, Xu Hui District, Shanghai, China, |
| Postcode: |
201108 |
| Tel: |
+86-21-54336592 |
| Fax: |
+86-21-54336593 |
| E-mail: |
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