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| Product Name: |
Recombinant Human Trypsin
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| Supply Ability: |
100000 |
| Related proudcts |
recombinant, trypsin, human, |
| Specifications |
1mg�0mg�00mg�g |
| Price Term: |
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| Port of loading: |
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| Minimum Order |
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Recombinant Human Trypsin
Catalog Number:RHT03
CAS: 9002-07-7
EC: 3.4.21.4
Storage temperature: 2-8â„?
Source: Recombinant human trypsin is a genetically engineered protein expressed in E.coli
Protein Sequence:The amino acid sequence of recombinant human trypsin is identical to human pancreas-derived trypsin
1.DESCRIPTION
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. YaxinBio recombinant human trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence is identical to human pancreas-derived trypsin,with equivalent properties co***red to native trypsin .The enzyme is inhibited by serine protease inhibitors, e.g. PMSF,soybean trypsin inhibitor and by metal chelating agents, e.g.EDTA.
2.MAIN FEATURES
Source Recombinant E. coli
Appearance White or off white powder
Specific activity �500 USP units/mg pro.
Protein Content �90%
Purity(SDS-PAGE) Single major band
Molecular Weight(SDS-PAGE) 24.0±2.4 kDa
Purity(HPLC) �95%
UNIT DEFINITION：One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25� with BAEE as a substrate (1cm light path).
3.RECOMMEND USAGE
Prepare 1-10mg/ml recombinant human trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.
4.STABILITY OF STORAGE AND TRANSPORT
�）Stability of storage: Recombinant human trypsin lyophilized should be stored under 2-8�in sealed container. It is stable within 24 months.After dissolved with 1mM HCl , it should be stored under -20�
It is no activity loss after 10 times repeated freezing and thawing.
�）Stability of transport: The product is stable by blue ice insulation transport.
5.PRODUCT ADVANTAGE
�）Animal origin free: Recombinant human trypsin is no exogenous virus contamination,and any animal origin material is not used in the product |
| Company: |
Shanghai Yaxin Biotechnology Co., Ltd.
|
| Contact: |
Mr. Zhi Zhao |
| Address: |
Room 101, Building 1, No. 466 Yin Du Road, Xu Hui District, Shanghai, China, |
| Postcode: |
201108 |
| Tel: |
+86-21-54336592 |
| Fax: |
+86-21-54336593 |
| E-mail: |
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