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| Product Name: |
Recombinant Carboxypeptidase B
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| Supply Ability: |
100000 |
| Related proudcts |
insulin, recombinant, |
| Specifications |
1mg�0mg�00mg |
| Price Term: |
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| Port of loading: |
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| Minimum Order |
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Recombinant Carboxypeptidase B
Catalog Number:RCPB01
CAS: 9025-24-5
EC: 3.4.17.2
Storage temperature: 2-8â„?
Source: Recombinant carboxypeptidase B is a genetically engineered protein expressed in E.coli
Protein Sequence:The amino acid sequence of recombinant carboxypeptidase B is identical to rat carboxypeptidase B
1.DESCRIPTION
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. YaxinBio has cloned rat carboxypeptidase B gene into E.coli for expression with equivalent properties co***red to animal original carboxypeptidase B .Recombinant carboxypeptidase B can replace native carboxypeptidase B for using in a variety of biotechnological processes.
2.MAIN FEATURES
Source Recombinant E. coli
Appearance White or off white, or yellowish powder
Specific activity �70 units/mg pro.
Purity(SDS-PAGE) Single major band
Molecular Weight(SDS-PAGE) 33.8±3.4 kDa
UNIT DEFINITION：One unit of carboxypeptidase B activity hydrolyzes one micromole ofhippuryl-L-arginine per minute at 25� pH 7.65.
3.RECOMMEND USAGE
Prepare 1-10mg/ml recombinant carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0.
4.STABILITY OF STORAGE AND TRANSPORT
�）Stability of storage: Recombinant carboxypeptidase B lyophilized should be stored under 2-8�in sealed container. It is stable within 24 months.After dissolved with sterile water or 25mM Tris-HCl, it should be stored under -20�
It is no activity loss after 10 times repeated freezing and thawing.
�）Stability of transport: The product is stable by blue ice insulation transport.
5.PRODUCT ADVANTAGE
①　Animal origin free: Recombinant carboxypeptidase B i |
| Company: |
Shanghai Yaxin Biotechnology Co., Ltd.
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| Contact: |
Mr. Zhi Zhao |
| Address: |
Room 101, Building 1, No. 466 Yin Du Road, Xu Hui District, Shanghai, China, |
| Postcode: |
201108 |
| Tel: |
+86-21-54336592 |
| Fax: |
+86-21-54336593 |
| E-mail: |
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