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| Product Name: |
recombinant trypsin
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| Supply Ability: |
100000 |
| Related proudcts |
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| Specifications |
colorless transparent liquid |
| Price Term: |
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Recombinant Trypsin
Catalog Number: RPT0201
CAS: 9002-07-7
EC: 3.4.21.4
Storage temperature: 2-8â„?
Source: Recombinant trypsin is a genetically engineered protein expressed in E.coli
Protein Sequence:The amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin
1.DESCRIPTION
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. YaxinBio recombinant trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties co***red to native trypsin .Recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes. The M.W. of recombinant trypsin is 24KD,and the optimum pH is pH 7.0-11.0. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF,soybean trypsin inhibitor and by metal chelating agents, e.g.EDTA.
2.MAIN FEATURES
Source Recombinant E. coli
Appearance White or off white, or yellowish powder
Specific activity �800 USP units/mg pro.
Purity(SDS-PAGE) Single major band
Molecular Weight(SDS-PAGE) 24.0±2.4 kDa
UNIT DEFINITION：One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25� with BAEE as a substrate (1cm light path).
3.RECOMMEND USAGE
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.
4.STABILITY OF STORAGE AND TRANSPORT
�）Stability of storage: Recombinant trypsin lyophilized should be stored under 2-8�in sealed container.It is stable within 24 months.After dissolved with 1mM HCl or 50mM HAC, it should be stored under -20�
It is no activity loss after 10 times repeated freezing and thawing.
�）Stability of transport: The product is stable by blue ice insulation transport.
5.PRODUCT ADVANTAGE
�）Animal origi |
| Company: |
Shanghai Yaxin Biotechnology Co., Ltd.
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| Contact: |
Mr. Zhi Zhao |
| Address: |
Room 101, Building 1, No. 466 Yin Du Road, Xu Hui District, Shanghai, China, |
| Postcode: |
201108 |
| Tel: |
+86-21-54336592 |
| Fax: |
+86-21-54336593 |
| E-mail: |
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