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| Product Name: |
Kex2 Protease
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| Supply Ability: |
1000000 |
| Related proudcts |
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| Specifications |
0.5U�0U�0U |
| Price Term: |
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| Port of loading: |
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Recombinant Kex2 Protease
Catalog Number: Re15
EC: 3.4.21.61
Synonyms: Endoproteinase Lys/Arg-Arg; Kex2 Protease;YSCF Protenase
Source: Recombinant Kex2 Protease is a genetically engineered protein expressed in Pichia pastoris
Storage temperature: 2-8â„?
Protein Sequence:The amino acid sequence of recombinant Kex2 Protease is identical to Saccharomyces cerevisiae Kex2
1.DESCRIPTION
Kex2 is a Ca2+ dependent serine protease and cleaves at C-terminal site of Lys-Arg, Arg-Arg, Pro-Arg in pro-α-factor and killer-toxin precursors maturing.But Kex2 can’t recognize and cut a single basic amino acid,such as carboxyl end peptide bond of arginine and lysine. Recombinant Kex2 protease is a genetically engineered protein expressed in Pichia pastoris and with equivalent properties co***red to native Saccharomyces cerevisiae Kex2. The activity of Kex2 is not affected by the conventional serine protease inhibitors such as PMSF,TPCK,TLCK inhibition.The optimal pH of Kex2 protease is 9.0. It is stable in buffer (pH 5.0-6.0).
2.MAIN FEATURES
Source Pichia pastoris
Appearance White or off white powder
Specific activity �.0 units/mg pro.
Purity(SDS-PAGE) Single major band
Molecular Weight(SDS-PAGE) 24.0±2.4 kDa
UNIT DEFINITION: One unit of Kex2 activity will release 1μmol 4-nitroaniline per minute in a reaction volume of 3.0 ml at pH8.0 and 25�with Boc-QRR-pNA (Boc-Gln-Arg-Arg-pNA) as the substrate.
3.RECOMMEND USAGEÂ
Recommended reaction buffer: pH 7.0-9.0, 50 mM Tris-HCl,2 mM Ca2 + or HEPES, 5 mM Ca2 +. If it is not used immediately after dissolving, it is recommended to dissolve the powder with 20 mM (pH 5.2) NaAc-HAc and 2 mM Ca2 + buffer to make the final concentration is about 1-10 mg/ml,then make suitable packing as your requirement and store at -20â„?Reaction was carried out, using pH 7.0-9.0, 50 mM Tris-HCl, 2 mM Ca2 + or HEPES, 5 mM Ca2 + buffer.
Note: The optimum reaction pH of the enzyme is pH 9.0 and the optimum stable pH is 5.0-6.0 |
| Company: |
Shanghai Yaxin Biotechnology Co., Ltd.
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| Contact: |
Mr. Zhi Zhao |
| Address: |
Room 101, Building 1, No. 466 Yin Du Road, Xu Hui District, Shanghai, China, |
| Postcode: |
201108 |
| Tel: |
+86-21-54336592 |
| Fax: |
+86-21-54336593 |
| E-mail: |
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