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| Product Name: |
sequencing grade trypsin
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| Supply Ability: |
100000 |
| Related proudcts |
trypsin, porcine trypsin, |
| Specifications |
100ug�mg |
| Price Term: |
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| Port of loading: |
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| Minimum Order |
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| Unit Price: |
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Sequencing Grade Modified Trypsin
Catalog Number:SRT0201
CAS:9002-07-7
EC:3.4.21.4
Storage temperature: 2-8â„?
Source: Sequencing grade modified trypsin is a genetically engineered methylation modified protein expressed in E.coli
Protein Sequence:The amino acid sequence of sequencing grade modified trypsin is identical to porcine pancreas-derived trypsin
1.DESCRIPTION
YaxinBio sequencing grade trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.
2.MAIN FEATURES
Source Recombinant E. coli
Appearance White or off white, or yellowish powder
Specific activity �500 USP units/mg pro.
SDS-PAGE Single major band
Molecular Weight(SDS-PAGE) 24.0±2.4 kDa
Purity(HPLC) �5%
3.RECOMMEND USAGE
It is recommended to dissolve or dilute with 50 mM HAc or 1 mM HCl. When used, dilute in 50 mM NH4HCO3 or ph7.0-8.0 buffer solution. 1 mM CaCl2 is recommended In the digestion buffer. The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-8.0.
4.STABILITY OF STORAGE AND TRANSPORT
�）Stability of storage: Sequencing grade trypsin lyophilized should be stored under 2-8�in sealed container. It is stable within 24 months.After dissolved with 1mM HCl or 50mM HAC, it should be stored under -20�
It is no activity loss after 5 times repeated freezing and thawing.
�）Stability of transport: The product is stable by blue ice insulation transport.
5.PRODUCT ADVANTAGE
�）Sequence grade recombinant protein: high specific |
| Company: |
Shanghai Yaxin Biotechnology Co., Ltd.
|
| Contact: |
Mr. Zhi Zhao |
| Address: |
Room 101, Building 1, No. 466 Yin Du Road, Xu Hui District, Shanghai, China, |
| Postcode: |
201108 |
| Tel: |
+86-21-54336592 |
| Fax: |
+86-21-54336593 |
| E-mail: |
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